Evaluation of Different Parasitological Techniques for Diagnosing Intestinal Parasites in Dogs

Read  full  paper  at:http://www.scirp.org/journal/PaperInformation.aspx?PaperID=53929#.VNxhWizQrzE

Intestinal nematodes are among the most common parasites of dogs, being Toxocara spp. and Ancylostoma spp. threats to public health due to their zoonotic potential. In this study, three parasitological diagnostic techniques for detecting the eggs/oocysts of canine intestinal parasites were evaluated. Fecal samples (n = 285) were collected from the environment (i.e. public streets, squares and homes) in Garanhuns, Pernambuco, Brazil. Samples were analyzed using the Willis-Mollay simple flotation technique, spontaneous sedimentation (Hoffman, Pons and Janer) and modified centrifugal flotation. Among the samples analyzed, 56.49% (161/285) showed eggs and/or oocysts of intestinal parasites. In particular, 44.21% (126/285) were detected by the Willis technique, 45.14% (121/268) by centrifugal flotation and 31.57% (90/285) by the Hoffmann technique. The kappa index indicated good concordance between the centrifugal flotation and Willis techniques (κ = 0.625), whereas fair concordance was observed between Hoffmann and Willis (κ = 0.480), and between Hoffman and centrifugal flotation (κ = 0.433). In conclusion, the centrifugal flotation technique showed high sensitivity towards detecting Ancylostoma spp. and Toxocara spp. (the most common parasites in this study) and could be used in association with the Willis technique to obtain safe diagnoses within routine laboratory analysis.

Cite this paper

de Santana, B. , da Silva, T. , Ramos, R. , Alves, L. and de Carvalho, G. (2015) Evaluation of Different Parasitological Techniques for Diagnosing Intestinal Parasites in Dogs. Open Journal of Veterinary Medicine, 5, 19-24. doi: 10.4236/ojvm.2015.52003.

References

[1] Otranto, D. and Eberhard, M.L. (2011) Zoonotic Helminths Affecting the Human Eye. Parasites & Vectors, 4, 41.
http://dx.doi.org/10.1186/1756-3305-4-41
[2] Santarém, V.A., Giuffrida, R. and Zanin, G.A. (2004) Larva migrans cutanea: Ocorrência de casos humanos e identificacao de larvas de Ancylostoma spp. em parque público do município de Taciba, Sao Paulo. Revista da Sociedade Brasileira de Medicina Tropical, 37, 179-181.
http://dx.doi.org/10.1590/S0037-86822004000200014
[3] Hohlenwerger, M.C., Almeida, M.A.O., Silva, A., Carvalho, S.M.S., Schilling, A.C. and Munhoz, A.D. (2011) Larvas de Ancilostomatídeos em sedimentos de solo de praias de Salvador, Bahia. Revista Brasileira de Medicina Veterinária, 33, 111-114.
[4] Andresiuk, M.V., Denegri, G.M., Esardella, N.H. and Hollmann, P. (2003) Encuesta coproparasitológico canina realizado em plazas publicas de la cuidad de Mar del Plata. Bueno Aires, Argentina. Parasitología Latinoamericana, 58, 17-22.
http://dx.doi.org/10.4067/S0717-77122003000100003
[5] Scaini, C.J., Tolado, R.N., Lovatel, R.R., Dionello, M.A., Gatti, F.A. and Susin, U.L. (2003) Contaminacao ambiental por ovos e larvas de helmintos em fezes de caes na área central do Balneário cassino, Rio Grande do Sul. Revista da Sociedade Brasileira de Medicina Tropical, 36, 529-619.
http://dx.doi.org/10.1590/S0037-86822003000500013
[6] Rey, L. (2008) Parasitologia: Parasitologia e Doencas Parasitárias do Homem nos Trópicos Ocidentais. 4th Edition, Guanabara Koogan, Rio de Janeiro.
[7] Menao, M.C., Faria, T.L.S.A., Almeida, J.M.S., Chiacchio, R.G. and Rocha, A. (2014) Pesquisa de infeccoes parasitárias com potencial zoonótico em animais mantidos em habitacoes coletivas. Atas de Saúde Ambiental, 2, 2-8.
[8] Santarém, V.A., Sartor, I.F. and Bergamo, F.M.M. (1998) Contamination by Toxocara spp. Eggs in Public Parks and Squares in Botucatu, Sao Paulo State, Brazil. Revista da Sociedade Brasileira de Medicina Tropical, 31, 529-532.
http://dx.doi.org/10.1590/S0037-86821998000600004
[9] Blazius, R.D., Emerick, S., Prophiro, J.S., Romao, P.R.T. and Silva, O.S. (2005) Ocorrência de protozoários e helmintos em amostras de fezes de caes errantes da cidade de Itapema, Santa Catarina. Revista da Sociedade Brasileira de Medicina Tropical, 38, 73-74.
http://dx.doi.org/10.1590/S0037-86822005000100018
[10] Campos Filho, P.C., Barros, L.M., Campos, J.O., Braga, V.B., Cazorla, I.M., Albuquerque, G.R. and Carvalho, S.M.S. (2008) Zoonotic Parasites in Dog Feces at Public Squares in the Municipality of Itabuna, Bahia, Brazil. Revista Brasileira de Parasitologia Veterinária, 17, 206-209.
[11] Mandarino-Pereira, A., de Souza, F.S., Lopes, C.W. and Pereira, M.J. (2010) Prevalence of Parasites in Soil and Dog Feces According to Diagnostic Tests. Veterinary Parasitology, 170, 176-181.
http://dx.doi.org/10.1016/j.vetpar.2010.02.007
[12] Sprenger, L.K., Green, K.T. and Molento, M.B. (2014) Geohelminth Contamination of Public Areas and Epidemiological Risk Factors in Curitiba, Brazil. Revista Brasileira de Parasitologia Veterinária, 23, 69-73.
http://dx.doi.org/10.1590/S1984-29612014009
[13] Núnez, F.A. (1999) Algunas consideraciones sobre el control de la calidad del diagnóstico de las parasitosis intestinales en América Latina. La Habana, 9, 257.
[14] Katagiri, S. and Oliveira-Sequeira, T.C.G. (2007) Zoonoses causadas por parasitas intestinais de caes e o problema do diagnóstico. Arquivos do Instituto Biológico, 74, 175-184.
[15] Papini, R., Campisi, E., Faggi, E., Pini, G. and Mancianti, F. (2012) Prevalence of Toxocara canis Eggs in Dog Faeces from Public Places of Florence, Italy. Helminthologia, 49, 154-158.
http://dx.doi.org/10.2478/s11687-012-0031-0
[16] Willis, H.H. (1921) A Simple Levitation Method for the Detection of Hookworm Ova. Medical Journal of Australia, 2, 375-376.
[17] Hoffman, W.A., Pons, J.A. and Janer, J.L. (1934) The Sedimentation Concentration Method in Schistosomiasis Mansoni. Puerto Rico Journal of Public Health and Tropical Medicine, 9, 283-289.
[18] Figueiredo, P.C., Serra-Freire, N.M. and Grisi, L. (1984) Eimerias de bovinos leiteiros no estado do Rio de Janeiro: Técnicas de diagnóstico e espécies identificadas. Atas da Sociedade de Biologia do Rio de Janeiro, 24, 22-26.
[19] Rey, L. (2002) Bases da Parasitologia Médica. 2nd Edition, Guanabara Koogan, Rio de Janeiro.
[20] Bowman, D.D. (2010) Georgis Parasitologia Veterinária. 9th Edition, Elsevier Brasil, Sao Paulo.
[21] Landis, J.R. and Kock, G.G. (1977) The Measurement of Observer Agreement for Categorical Date. Biometrics, 33, 159-174. http://dx.doi.org/10.2307/2529310
[22] Cerqueira, E.J.L., Arcanjo, M.S. and Alcantara, L.M. (2007) Análise comparativa da sensibilidade da técnica de Willis no diagnóstico parasitológico da ancilostomíase. Dialogos & Ciência, 5, 1-7.
[23] Mergener, D., Caricimo, A., Langer, D., Klein, D., Lima, G., Verona, J., et al. (2013) Enteroparasitos de caes errantes da zona urbana dos municípios de água Doce, Irani, Joacaba, Ponte Serrada e Treze Tílias, Santa Catarina, Brasil. Unoesc & Ciência, 4, 127-134.                                                     eww150212lx
[24] Sloss, W.M., Zajac, M.A. and Kemp, R.L. (1999) Parasitologia clínica veterinária. Manole, Sao Paulo.
Advertisements

发表评论

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out / 更改 )

Twitter picture

You are commenting using your Twitter account. Log Out / 更改 )

Facebook photo

You are commenting using your Facebook account. Log Out / 更改 )

Google+ photo

You are commenting using your Google+ account. Log Out / 更改 )

Connecting to %s