High Levels Production of Recombinant Human Activin A—Effect upon in Vivo Follicle Stimulation

Read  full  paper  at:http://www.scirp.org/journal/PaperInformation.aspx?PaperID=54066#.VOBb7CzQrzE

Activin plays an important role in numerous physiological processes such as cell differentiation and remodeling, regeneration and repair of tissues from various organs, angiogenesis, morphogenesis of glandular organs, pluripotency and differentiation of stem cells, cell adhesion and apoptosis. It participates in reproductive processes like embryogenesis, in the expression of Follicle Stimulating Hormone and Luteinizing Hormone and maturation of ovarian follicles and therefore has application in the area of reproduction of vertebrates. Given the economic importance of activin, we develop an efficient and economical method for the production of recombinant human activin A (rACT), using as expression system the yeast Pichia pastoris. rACT showed biological activity as it induced, on submicromolar dose, the increase of ovarian mass and the ovulation process in a mammal model.

Cite this paper

de Souza, N. , de Almeida, M. , Walkiria de Araujo, G. , de Melo Leite, R. , Santos, I. , Lima, M. and Pesquero, J. (2015) High Levels Production of Recombinant Human Activin A—Effect upon in Vivo Follicle Stimulation. Advances in Bioscience and Biotechnology, 6, 96-104. doi: 10.4236/abb.2015.62010.

References

[1] Ling, N., et al. (1986) Pituitary FSH Is Realeased by a Heterodimer of the β-Subunits from the Two Forms of Inhibin. Nature, 321, 779-782.
http://dx.doi.org/10.1038/321779a0
[2] Vale, W., Hsueh, A., Rivier, C. and Yu, J. (1990) Theinhibin/Activin Family of Hormones and Growth Factors. Handbook of Experimental Pharmacology, 95, 211-248.
http://dx.doi.org/10.1007/978-3-642-74781-6_8
[3] Nakamura, T., et al. (1992) Isolation and Characterization of Native Activin B. Journal of Biological Chemistry, 267, 16385-16389.
[4] Burger, H.G. and Igarashi, M. (1988) Inhibin: Definition and Nomenclature, including Related Substances. Endocrinology, 122, 1701-1702.
http://dx.doi.org/10.1210/endo-122-4-1701
[5] Roberts, V.J., Peto, C.A., Vale, W. and Sawchenko, P.E. (1992) Inhibin/Activin Subunits Are Co-Stored with FSH and LH in Secretory Granules of the Rat Anterior Pituitary Gland. Neuroendocrinology, 56, 214-224.
http://dx.doi.org/10.1159/000126231
[6] Luisi, S., Florio, P., Reis, F.M. and Petraglia, F. (2001) Expression and Secretion of Activin-A: Possible Physiological and Clinical Implications. European Journal of Endocrinolgy, 145, 225-236.
http://dx.doi.org/10.1530/eje.0.1450225
[7] Jones, R.L., et al. (2002) Expression of Activin Receptors, Foliistatin and Betaglycan by Human Endometrial Stromal cells Consist with a Role for Activins during Decidualization. Molecular Human Reprodroduction, 8, 363-374.
http://dx.doi.org/10.1093/molehr/8.4.363
[8] Hongisto, H., Mikhailova, A., Hiidenmaa, H., Ilmarinen, T. and Skottman, H. (2012) Low Level of Activin Secreted by Fibroblast Feeder Cells Accelerates Early Stage Differentiation of Retinal Pigment Epitelial Cells from Human Pluripotent Stem Cells. Stem Cell Discovery, 4, 176-186.
http://dx.doi.org/10.4236/scd.2012.24022
[9] Hübner, G., Alzheimer, C. and Werner, S. (1999) Activin A: A Novel Player in Tissue Repair Process. Histology and Histopathology, 14, 295-304.
[10] Smith, J.C., Price, B.M., Van Nimmen, K. and Huylebroeck, D. (1990) Identification of a Potent Xenopus Mesoderm-Inducing Factor as a Homologue of Activin A. Nature, 345, 729-731.
http://dx.doi.org/10.1038/345729a0
[11] Maeshima, K., Maeshima, A., Hayashi, Y., Kishi, S. and Kojima, I. (2004) Crucial Role of Activin A in Tubulogênesis of Endothelial Cells Induced by Vascular Endothelial Growth Factor. Endocrinology, 145, 3739-3745.
http://dx.doi.org/10.1210/en.2004-0213
[12] Werner, S. and Alzheimer, C. (2006) Roles of Activin in Tissue Repair, Fibrosis, and Inflammatory Disease. Cytokine & Growth Factor Reviews, 17, 157-171.
http://dx.doi.org/10.1016/j.cytogfr.2006.01.001
[13] Chen, A.E., Borowiak, M., Sherwood, R.I., Kweudjeu, A. and Melton, D.A. (2013) Functional Evaluation of ES Cell-Derived Endodermal Populations Reveals Differences between Nodal and Activin A-Guided Differentiation. Development, 140, 675-686.
[14] Suszko, M.I., Balkin, D.M., Chen, Y. and Woodruff, T.K. (2005) Smad3 Mediates Activin-Induced Transcription of Follicle-Stimulating Hormone Beta-Subunit Gene. Molecular Endocrinology, 19, 1849-1858.
http://dx.doi.org/10.1210/me.2004-0475
[15] Knight, P.G. and Glister, C. (2006) TGF-Beta Superfamily Members and Ovarian Follicle Development. Reproduction, 132, 191-206.
http://dx.doi.org/10.1530/rep.1.01074
[16] Woodruff, T.K., Lyon, R.J., Hansen, S.E., Rice, G.C. and Mather, J.P. (1990) Inhibin and Activin Locally Regulate Rat Ovarian Folliculogenesis. Endocrinology, 127, 3196-3205.
http://dx.doi.org/10.1210/endo-127-6-3196
[17] Vale, W., Bilezikjian, L.M. and Rivier, C. (1994) Reproductive and Other Roles of Inhibins and Activins. In: The Physiology of Reproduction, 2nd Edition, Raven Press Ltd., New York, 1861-1878.
[18] Li, R., Phillips, D.M. and Mather, J.P. (1995) Activin Promotes Ovarian Follicle Development in Vitro. Endocrinology, 136, 849-856.
[19] Mader, A., Chromikova, V. and Kunert, R. (2013) Recombinant IgM Expression in Mammalian Cells: A Target Protein Challenging Biotechnological Production. Advances in Bioscience and Biotechnology, 4, 38-43.
[20] Damasceno, L.M., Huang, C. and Batt, C.A. (2012) Protein Secretion in Pichia pastoris and Advances in Protein Production. Applied Microbiology and Biotechnology, 93, 31-39.
[21] Shibui, T., Bando, K. and Misawa, S. (2013) High-Level Secretory Expression, Purification, and Characterization of an Anti-Human Her II Monoclonal Antibody, Trastuzumab, in the Methylotrophic Yeast Pichia pastoris. Advances in Bioscience and Biotechnology, 4, 640-646.
http://dx.doi.org/10.4236/abb.2013.45084
[22] Zapatero-Caballero, H., Sanchez-Franco, F., Fernandez-Mendez, C., García-San Frutos, M., Botella-Cubells, L.M. and Fernandez-Vazquez, G. (2004) Gonadotropin-Releasing Hormone Receptor Gene Expression during Pubertal Development of Female Rats. Biology of Reproduction, 70, 348-355.
http://dx.doi.org/10.1095/biolreprod.103.020818
[23] Chen, Y., Cino, J., Hart, G., Freedman, D., White, C. and Ko-mives, E.A. (1997) High Protein Expression in Fermentation of Recombinant Pichia pastoris by a Fed-Batch Process. Process Biochemistry, 32, 107-111.
http://dx.doi.org/10.1016/S0032-9592(96)00052-0
[24] Bradford, M.M. (1976) A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding. Analytical Biochemistry, 72, 248-254.
http://dx.doi.org/10.1016/0003-2697(76)90527-3
[25] Papakonstantinou, T., Harris, S.J., Fredericks, D., Harrison, C., Wallace, E.M. and Hearn, M.T.W. (2009) Synthesis, Purification and Bioactivity of Recombinant Human Activin A Expressed in the Yeast Pichia pastoris. Protein Expression and Purification, 64, 131-138.
http://dx.doi.org/10.1016/j.pep.2008.10.020
[26] Fredericks, D., Clay, R., Warner, T., O’Connor, A., Kretser, D.M. and Hearn, M.T.W. (2010) Optimization of the Expression of Recombinant Human Activin A in the Yeast Pichia pastoris. Biotechnology Progress, 26, 372-383.
http://dx.doi.org/10.1002/btpr.304
[27] Cereghino, G.P., Cereghino, J.L., Ilgen, C. and Cregg, J.M. (2002) Production of Recombinant Proteins in Fermenter Cultures of the Yeast Pichia pastoris. Current Opinion in Biotechnology, 13, 329-332.
http://dx.doi.org/10.1016/S0958-1669(02)00330-0
[28] Cregg, J.M., Cereghino, J.L., Shi, J. and Higgins, D.R. (2000) Recombinant Protein Expression in Pichia pastoris. Molecular Biotechnology, 16, 23-52.
http://dx.doi.org/10.1385/MB:16:1:23
[29] Pangas, S.A. and Woodruff, T.K. (2002) Production and Purification of Recombinant Human Inhibin and Actvin. Journal of Endocrinology, 172, 199-210.
http://dx.doi.org/10.1677/joe.0.1720199
[30] Hirshfield, A.N. (1991) Development of Follicles in the Mammalian Ovary. International Review of Cytology, 124, 43-101.
http://dx.doi.org/10.1016/S0074-7696(08)61524-7
[31] Richards, J.S., Russell, D.L., Ochsner, S., Hsieh, M., Doyle, K.H., Falender, A.E., Lo, Y.K. and Sharma, S.C. (2002) Novel Signaling Pathways that Control Ovarian Follicular Development, Ovulation, and Luteinization. Recent Progress in Hormone Research, 57, 195-220.
http://dx.doi.org/10.1210/rp.57.1.195
[32] Wang, W., Tang, Y., Ni, L., Jongwutiwes, T., Liu, H. and Rosenwaks, Z. (2012) A Modified Protocol for in Vitro Maturation of Mouse Oocytes from Secondary Preantral Follicles. Advances in Bioscience and Biotechnology, 3, 57-74.
[33] Erickson, G.F., Kokka, S. and Rivier, C. (1995) Activin Causes Premature Superovulation. Endocrinology, 136, 4804-4813.
[34] Cossigny, D.A., Findlay, J.K. and Drummond, A.E. (2012) The Effects of FSH and Activin A on Follicle Development in Vitro. Reproduction, 143, 221-229.
http://dx.doi.org/10.1530/REP-11-0105
[35] Ethier, J.F. and Findlay, J.K. (2001) Roles of Activin and Its Signal Transduction Mechanisms in Reproductive Tissues. Reproduction, 121, 667-675.
http://dx.doi.org/10.1530/rep.0.1210667
[36] Suszko, M.I., Lo, D.J., Suh, H., Camper, S.A. and Woodruff, T.K. (2003) Regulation of the Rat Follicle-Stimulating Hormone Beta-Subunit Promoter by Activin. Molecular Endocrinology, 17, 318-332.
http://dx.doi.org/10.1210/me.2002-0081
[37] Burger, L.L., Haisenleder, D.J., Dalkin, A.C. and Marshall, J.C. (2004) Regulation of Gonadotropin Subunit Gene Transcription. Journal of Molecular Endocrinology, 33, 559-584.
http://dx.doi.org/10.1677/jme.1.01600
[38] Gregory, S.J., Lacza, C.T., Detz, A.A., Xu, S., Petrillo, L.A. and Kaiser, U.B. (2004) Synergy between Activin A and Gonadotropin-Releasing Hormone in Transcriptional Activation of the Rat Follicle-Stimulating Hormone Beta Gene. Molecular Endocrinology, 19, 237-254.
http://dx.doi.org/10.1210/me.2003-0473
[39] Lamba, P., Santos, M.M., Philips, D.P. and Bernard, D.J. (2006) Acute Regulation of Murine Follicle-Stimulating Hormone Beta Subunit Transcription by Activin A. Journal of Molecular Endocrinology, 36, 201-220.
http://dx.doi.org/10.1677/jme.1.01961
[40] Thackray, V.G., Mellon, P.L. and Coss, D. (2010) Hormones in Synergy: Regulation of the Pituitary Gonadotropin Genes. Molecular and Cellular Endocrinology, 314, 192-203.
[41] Bernard, D.J. and Tran, S. (2013) Mechanisms of Activin-Stimulated FSH Synthesis: The Story of a Pig and a FOX. Biology of Reproduction, 88, 78.
http://dx.doi.org/10.1095/biolreprod.113.107797
[42] Doi, M., Igarashi, M., Hasegawa, Y., Eto, Y., Shibai, H., Miura, T. and Ibuki, Y. (1992) In Vivo Action of Activin-A on Pituitary-Gonadal System. Endocrinology, 130, 139-144.
[43] Woodruff, T.K. and Mather, J.P. (1995) Inhibin, Activin and the Female Reproductive Axis. Annual Review of Physiology, 57, 219-244.
http://dx.doi.org/10.1146/annurev.ph.57.030195.001251
[44] Gajewska, A., Siawrys, G., Bogacka, I., Przala, J., Lerrant, Y., Counis, R. and Kochman, K. (2002) In Vivo Modulation of Follicle-Stimulating Hormone Release and Beta Subunit Gene Expression by Activin A and the GnRH Agonist Buserelin in Female Rats. Brain Research Bulletin, 58, 475-480.
http://dx.doi.org/10.1016/S0361-9230(02)00821-3
[45] Thomas, F.H., Armstrong, D.G. and Telfer, E.E. (2003) Activin Promotes Oocyte Development in Ovine Preantral Follicles in Vitro. Reproductive Biology and Endocrinology, 1, 76.
[46] Silva, J.R., van den Hurk, R., van Tol, H.T.A., Roelen, B.A.J. and Figueiredo, J.R. (2004) Gene Expression and Protein Localisation for Activin-A, Follistatin and Activin Receptors in Goat Ovaries. Journal of Endocrinology, 183, 405-415.
http://dx.doi.org/10.1677/joe.1.05756                                  eww150215lx
Advertisements

发表评论

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out / 更改 )

Twitter picture

You are commenting using your Twitter account. Log Out / 更改 )

Facebook photo

You are commenting using your Facebook account. Log Out / 更改 )

Google+ photo

You are commenting using your Google+ account. Log Out / 更改 )

Connecting to %s