Genetic Variations and Cultivar Purity within the G. hirsutum Cultivars via RAPD Marker

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To assess genetic variation within a cultivar, four commercial varieties with five individual G. hirsutum genotypes and their bulk were assayed with selected forty 10-mer RAPDs per cultivar via PCR. CIM-473 cultivar showed amplification of 321 bands in total per primer with an average of 8 bands. A range of similarity i.e.; 80% – 100% within the cultivar has been observed for minimum and maximum respectively. CIM-448 showed amplification of 300 bands in total per primer with an average of 7.5 bands. Maximum similarity of 95% and minimum similarity of 84% was observed within the cultivar. In CIM-1100 the total numbers of fragments amplified are 304, with the average of 7.6 bands per primer and are 12% – 20% polymorphic. Similarly, in NIAB-78 the total numbers of fragments amplified were 310, with the average of 7.7 bands per primer. The reliability of RAPD technique for finding genetic resemblances is indicated by the mentioned results. Numerous groups within 5 individual genotypes bulk among 4 cultivars of G. hirsutum L. have been through cluster analysis which was by the unweighted pair group method of arithmetic means (UPGMA). Dendrograms have been used for showing cultivar relationship.

Cite this paper

Raheel, F. , Shah, M. , Iqbal, M. , Noorka, I. and Sarfraz, Z. (2015) Genetic Variations and Cultivar Purity within the G. hirsutum Cultivars via RAPD Marker. American Journal of Plant Sciences, 6, 392-404. doi: 10.4236/ajps.2015.62045.

References

[1] Iqbal, M.J., Aziz, N., Saeed, N.A., Zafar, Y. and Malik, K.A. (1997) Genetic Diversity of Some Elite Cotton Varieties by RAPD Analysis. Theoretical and Applied Genetics, 94, 139-144.
[2] Tatineni, V., Cantrell, R.G. and Davis, D.D. (1996) Genetic Diversity in Elite Cotton Germplasm Determined by Morphological Characteristics and RAPDs. Crop Sciecne, 36, 186-192.
[3] Linos, A.A., Bebeli, P.J. and Kaltsikes, P.J. (2002) Cultivar Identification in Upland Cotton Using RAPD Markers. Australian Journal of Agriculture Research, 53, 637-642.
http://dx.doi.org/10.1071/AR01129
[4] Raheel, F., Saima, S., Dasti, A.A. and Mahmood, Z. (2005) Morpho-Taxonomic Study of Some Old World Cotton Species. Pakistan Journal of Plant Science, 11, 45-50.
[5] Sudhanshu, J. (1997) Studies on Combining Ability in Intra and Interspecific Crosses of Cotton. Crop Research Hisar, 14, 91-95.
[6] Lerner, I.M. (1954) Genetic Homeostasis. John Wiley and Sons, New York.
[7] Gustafsson, A. (1948) The Effect of Heterozygosity on Variability and Vigour. Hereditos, 32, 263-286. http://dx.doi.org/10.1111/j.1601-5223.1946.tb02779.x
[8] Zhu, Y., Chen, H., Fan, J., Wang, Y., Li, Y., Chen, J., Fan, J., Yang, S., Hu, L., Leung, H., Mew, T.W., Teng, P.S., Wang, Z. and Mundt, C.C. (2000) Genetic Diversity and Disease Control in Rice. Nature, 406, 718-722.
http://dx.doi.org/10.1038/35021046
[9] Rommens, J.M., Iannuzzi, M.C., Kerem, B., Drumm, M.L., Melmer, G., Dean, M., Rozmahel, R., Cole, J., Kennedy, D., Hidaka, N., Zsiga, M., Buchwald, M., Riordan, J.R., Tsui, L.C. and Collins, F.S. (1989) Identification of the Cystic Fibrosis Gene: Chromosome Walkihg and Jumping. Science, 245, 1059-1065.
http://dx.doi.org/10.1126/science.2772657
[10] Wallace, M.R., Marchuk, D.A., Anderson, R., Letcher, R., Odeh, H.M., Saulino, A.M., Fountain, J.W., Bereton, A., Nicholson, J., Mitchell, A.L., Brownstein, B.H. and Collins, F.S. (1990) Type 1 Neurofibromatosis Gene: Identification of a Large Transcript Disrupted in Three NF1 Patients. Science, 249, 181-186.
[11] Paterson, A.H., De Verna, J.W., Lanini, B. and Tanksley, S.D. (1990) Fine Mapping of Quantitative Trait Loci Using Selected Overlapping Recombinant Chromosomes, in an Interspecies Cross of Tomato. Genetics, 124, 735-742.
[12] Bowman, D.T., May, O.L. and Calhoun, D.S. (1996) Genetic Base of Upland Cotton Cultivars Released between 1970 and 1990. Crop Science, 36, 577-581.
http://dx.doi.org/10.2135/cropsci1996.0011183X003600030008x
[13] Iqbal, M.J. and Rayburn, A.L. (1994) Stability of RAPD Markers for Determining Cultivar-Specific DNA Profiles in Rye (Seeale cereal L.). Euphytica, 75, 215-220. http://dx.doi.org/10.1007/BF00025606
[14] Doyle, J.J. and Doyle, J.H. (1990) Isolation of Plant DNA from Fresh Tissue. Focus, 12, 13-15.
[15] Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual. 2nd Edition, Cold Spring Harbor Laboratory, Cold Spring Harbor.
[16] Williams, C.E. and St. Clair, D.A. (1993) Phenetic Relationships and Levels of Variability Detected by Restriction Fragment Length Polymorphism and Random Amplified DNA Analysis of Cultivated and Wild Accessions of Lycopersicon esculentum. Genome, 36, 619-630.
http://dx.doi.org/10.1139/g93-083
[17] Maniatis, T., Fritsch, E.F. and Sambrook, J. (1982) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor.
[18] Sokal, R.R. and Sneath, P.H.A. (1963) Principles of Numerical Taxonomy. W.H. Freeman, San Francisco.
[19] Rani, V., Parida, A. and Raina, S.N. (1995) Random Amplified Polymorphic DNA (RAPD) Markers for Genetic Analysis in Micropropagated Plants of Populus deltoides Marsh. Plant Cell Reports, 14, 459-462.
http://dx.doi.org/10.1007/BF00234055
[20] Soniya, E.V., Banerjee, N.S. and Das, M.R. (2001) Genetic Analysis of Somaclonal Variation among Callus-Derived Plants of Tomato. Current Science, 80, 1213-1215.
[21] Thormann, C.E., Ferreira, M.E., Camargo, L.E.A., Tivang, J.G. and Osborn, T.C. (1994) Comparison of RFLP and RAPD Markers to Estimating Genetic Relationship within and among Cruciferous Species. Theoretical and Applied Genetics, 88, 973-980.
http://dx.doi.org/10.1007/BF00220804
[22] Fouilloux, G. and Bannerot, H. (1988) Selection Methods in Common Bean (Phaseolus vulgaris L.). In: Gepts, P., Ed., Genetic Resources of Phaseolus Beans, Klüwer Academic Publishers, Dordrecht, 503-541.
http://dx.doi.org/10.1007/978-94-009-2786-5_21
[23] Franca, L.T., Carrilho, E. and Kist, T.B. (2002) A Review of DNA Sequencing Techniques. Quarterly Reviews of Biophysics, 35, 169-200. http://dx.doi.org/10.1017/S0033583502003797
[24] Multani, D.S. and Lyon, B.R. (1995) Genetic Fingerprinting of Australian Cotton Cultivars with RAPD Markers. Genome, 38, 1005-1008. http://dx.doi.org/10.1139/g95-132
[25] Nie, Y.C., Zuo, K.J., Zhang, X.L., Feng, C.D. and Liu, J.L. (2000) Application of RAPD Markers in Differentiating Germplasm Lines from 4x Gossypium arboreum x G. anomalum x G. hirsutum. Scientia Agricultura Sinica, 33, 25- 29.
[26] Vas-Esbroeck, G.A. and Bowman, D.T. (1998) Cotton Germplasm Diversity and Its Importance to Cultivar Development. Journal of Cotton Sciences, 2, 121-129.
[27] Tokatlidis, I.S., Tsikrikoni, C., Tsialtas, J.T., Lithourgidis, A.S. and Bebeli, P.J. (2008) Variability within Cotton Cultivars for Yield, Fiber Quality and Physiological Traits. The Journal of Agricultural Science, 146, 483-490. http://dx.doi.org/10.1017/S0021859608007867
[28] Jain, A., Bhatta, S., Banga, S.S., Prakash, S. and Lakshmikumaran, M. (1994) Potential Use of Random Amplified Polymorphic DNA (RAPD) Technique to 201 Study the Genetic Diversity in Indian Mustard (Brassica juncea) and Its Relationship to Heterosis. Theoretical and Applied Genetics, 88, 116-122.
http://dx.doi.org/10.1007/BF00222403
[29] Tafvizi, F., Sheidai, M., Farahanei, F., Nourmohammadi, Z. and Alishah, O. (2010) Somaclonal Variation in Three Tetraploid Cotton (Gossypium hirsutum L.) Cultivars. Cotton Research Center, Gorgan.   eww150225lx
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