Histological and Histochemical Studies on the Early Developmental Stages of the Egyptian Toad Bufo regularis Reuss

Read  full  paper  at: http://www.scirp.org/journal/PaperInformation.aspx?PaperID=55362#.VSORufzLfHE

The present study was carried out to investigate the histological and histochemical changes in the liver and skin on different developmental stages of Egyptian toad Bufo regularis to be used as a histological key for such species. Our experiment started when tadpoles began to feed. The adapted embryos are divided into 3 large tanks of 200 embryos each, collections of samples started from feeding age every three days. Both histological and histochemical results showed that the general architecture of the different organs was correlated with the state of development, i.e. larval, met-amorphic and post-metamorphic. They, therefore, displayed different characteristic features de-pending on the investigated developmental stage starting from the larval stage (stage 44) and ending with the post-metamorphic stage 66.

Cite this paper

Sayed, A. , Elballouz, A. and Wassif, E. (2015) Histological and Histochemical Studies on the Early Developmental Stages of the Egyptian Toad Bufo regularis Reuss. Open Journal of Animal Sciences, 5, 142-156. doi: 10.4236/ojas.2015.52017.


[1] Wester, P.W. and Canton, J.H. (1991) The Usefulness of Histopathology in Aquatic Toxicity Studies. Comparative Biochemistry and Physiology, 100, 115-117.
[2] Thophona, S., Kruatrachuea, M., Upathama, E.S., Pokethitiyooka, P., Sahaphongb, S. and Jaritkhuanc, S. (2003) Histopathological Alterations of White Seabass, Lates calcarifer in Acute and Subchronic Cadmium Exposure. Environmental Pollution, 121, 307-320.
[3] Scharfettner-Kochanek, K., Wlaschek, M., Brenneisen, P., Schauen, M., Blaudschun, R. and Wenk, J. (1997) UV-Induced Reactive Oxygen Species in Photocarcinogenesis and Photoageing. Journal of Biological Chemistry, 378, 1247-1257.
[4] Gernhofer, M., Pawet, M., Schramm, M., Müller, E. and Triebskorn, R. (2001) Ultrastructural Biomarkers as Tools to Characterize the Health Status of Fish in Contaminated Streams. Journal of Aquatic Ecosystem Stress and Recovery, 8, 241-226.
[5] Akiyoshi, H. and Inoue, A.M. (2012) Comparative Histological Study of Hepatic Architecture in the Three Orders Amphibian Liver. Comparative Hepatology, 11, 1-8.
[6] Hoffman, J. and Katz, U. (1998) Glyconeogenesis and Urea Synthesis in the Toad Bufo viridis during Acclimation to Water Restriction. Physiological Zoology, 71, 85-92.
[7] Osman, A.H., Pfeiffer, C.J. and Asashima, M. (1991) Liver Uhrastructure and a New Cell Type in the Japanese Newt, Cynops pyrrhogaster. European Journal of Morphology, 29, 255-270.
[8] Goldblatt, P.J., Hampton, J.A., DiDio, L.N., Skeel, K.A. and Klaunig, J.E. (1987) Morphologic and Histochemical Analysis of the Newt (Notophthalmus viridescens) Liver. The Anatomical Record, 217, 328-338.
[9] Rappaport, A.M. (1967) Diseases of the Liver. In: Schiff, L., Ed., Anatomic Considerations, 2nd Edition, Lippincott Company—Asian Edition Hakko Co. Ltd., Philadelphia, 1-46.
[10] Menon, J. and Wahrman, M. (2001) Ultrastructure Observations on Effect of Different Concentrations of Calicum and Thyroxine in Vitro and Larval Epidermal Cells of Rana catesbelana Tadpoles. In Vitro Cellular & Developmental Biology—Animal, 37, 283-292.


Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out /  更改 )

Google+ photo

You are commenting using your Google+ account. Log Out /  更改 )

Twitter picture

You are commenting using your Twitter account. Log Out /  更改 )

Facebook photo

You are commenting using your Facebook account. Log Out /  更改 )


Connecting to %s